Leptospira in Slaughtered Fattening Pigs in Southern Italy: Serological Survey and Molecular Typing

Autor: Giusi Macaluso, Alessandra Torina, Valeria Blanda, Annalisa Guercio, Antonio Lastra, Ilenia Giacchino, Rosalia D’Agostino, Carmela Sciacca, Mario D’Incau, Cristina Bertasio, Francesca Grippi
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Animals, Vol 12, Iss 5, p 585 (2022)
Druh dokumentu: article
ISSN: 2076-2615
DOI: 10.3390/ani12050585
Popis: Leptospirosis is a re-emerging zoonosis of worldwide significance; a wide spectrum of wild and domestic animal species act as natural or accidental hosts. Swine can act as maintenance or accidental hosts of pathogenic Leptospira spp. This study aimed at investigation of Leptospira spp. prevalence and diversity in slaughtered pigs in southern Italy (Sicily). In total, 55 samples of kidneys and blood were collected. Microscopic agglutination test and real-time PCR were performed to detect pathogenic and intermediately pathogenic Leptospira. Partial rpoB gene sequencing and multi-locus sequence typing (MLST) were performed to characterize Leptospira species. The analysis showed a seropositivity rate of 16.4%, with Australis representing the most frequently identified serogroup (63.64%); Pomona and Sejroe were detected with a prevalence of 27.27% and 9.09%, respectively. Pathogenic Leptospiral DNA was detected in 2 kidney samples (3.64%). Leptospira were identified through MLST as L. borgpetersenii serovar Tarassovi (serogroup Tarassovi). Obtained data confirmed the presence of Leptospira infection among pigs in southern Italy, suggesting that management of these animals may be considered an occupational risk for humans.
Databáze: Directory of Open Access Journals
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