An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states

Autor: Caterina Alfano, Domenico Sanfelice, Stephen R. Martin, Annalisa Pastore, Piero Andrea Temussi
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/ncomms15428
Popis: Crowding effects—important when considering cellular environments—greatly influence protein stability. Here the authors study the impact of macromolecular crowders on high and low temperature protein unfolding, and show that volume exclusion effects are larger when the protein and crowder volumes are similar.
Databáze: Directory of Open Access Journals