| Popis: |
Diabetes is a chronic metabolic disorder, as well as a situation of increased oxidative stress. We examined the distribution of somatostatin in gastric tissues of cinnamon extract treated streptozotocin-induced diabetic rats using the immunohistochemistry technique. A total of 30 male Sprague Dawley rats were used in the study. The rats were assigned to five groups as control, sham, cinnamon, diabetes and diabetes + cinnamon. No application was made to the control group, the sham group received intraperitoneally (i.p.) 50 mg/kg sodium citrate, and diabetes was induced by i.p. injection of 50 mg/kg STZ in diabetes and diabetes + cinnamon groups. Cinnamon extracts were then given to cinnamon and diabetes + cinnamon groups by oral gavage at a dose of 200 mg/kg for 14 days. The streptavidin-biotin-peroxidase method was used to determine the immunoreactivity of somatostatin. Gastric tissue sections were prepared and stained by Crossman"s triple and Hematoxylin-Eosin staining in order to examine histological structure of the gastric tissue. We determined that somatostatin immunoreactivity of the control, sham and cinnamon groups was stronger than for the diabetes, and diabetes + cinnamon groups. While a weak immunoreactivity was found in the cardia, fundus and pyloric mucosa of the gastric tissue in the diabetes and diabetes + cinnamon groups, a strong immunoreactivity was found in the cinnamon, sham, and control groups. Also, a statistically significant was observed when all groups compared in terms of count of parietal and principal cells (P |
