| Autor: |
H Kadowaki, E G Bremer, J E Evans, F B Jungalwala, R H McCluer |
| Jazyk: |
angličtina |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 24, Iss 10, Pp 1389-1397 (1983) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)37890-1 |
| Popis: |
An improved method for the hydrolysis of long chain bases from gangliosides with aqueous acetonitrile-HCl is described. The long chain bases released from brain gangliosides were derivatized with biphenylcarbonylchloride and resolved by high performance liquid chromatography on a C18 reversed-phase column. Components of individual peaks were identified by gas-liquid chromatography-mass spectrometry as their trimethylsilyl derivatives. The acetonitrile-HCL hydrolysis procedure resulted in no formation of O-methyl ethers of long chain bases and a significant decrease in the level of secondary products. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
