Circulating histone H3 levels are increased in septic mice in a neutrophil-dependent manner: preclinical evaluation of a novel sandwich ELISA for histone H3

Autor:	Takashi Ito, Mayumi Nakahara, Yoshiki Masuda, Sachie Ono, Shingo Yamada, Hiroyasu Ishikura, Hitoshi Imaizumi, Chinatsu Kamikokuryo, Yasuyuki Kakihana, Ikuro Maruyama
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Histone Sepsis Neutrophil ELISA Medical emergencies. Critical care. Intensive care. First aid RC86-88.9
Zdroj:	Journal of Intensive Care, Vol 6, Iss 1, Pp 1-6 (2018)
Druh dokumentu:	article
ISSN:	2052-0492
DOI:	10.1186/s40560-018-0348-y
Popis:	Abstract Background Nuclear histone proteins are released into the extracellular space during sepsis and act as major mediators of death. However, circulating histone levels have not been precisely quantified. Methods We developed a novel enzyme-linked immunosorbent assay (ELISA) for detection of circulating histone H3 levels and evaluated its performance. Using the ELISA, we measured plasma histone H3 levels in C57BL/6 J mice subjected to cecal ligation and puncture (CLP)-induced sepsis. Results The newly developed ELISA enabled reproducible measurement of histone H3 levels with a working range up to 250 ng/mL. Using the ELISA, we found that plasma histone H3 levels were elevated in septic mice compared with sham-operated mice (p
Databáze:	Directory of Open Access Journals
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