Extracellular vesicles yield predictive pre-eclampsia biomarkers

Autor: Kok Hian Tan, Soon Sim Tan, Mor Jack Ng, Wan Shi Tey, Wei Kian Sim, John Carson Allen, Sai Kiang Lim
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of Extracellular Vesicles, Vol 6, Iss 1 (2017)
Druh dokumentu: article
ISSN: 2001-3078
20013078
DOI: 10.1080/20013078.2017.1408390
Popis: Circulating extracellular vesicles (EVs) such as cholera toxin B chain (CTB)- or annexin V (AV)-binding EVs were previously shown to be rich sources of biomarkers. Here we test if previously identified pre-eclampsia (PE) candidate biomarkers, TIMP-1 in CTB-EVs (CTB-TIMP) and PAI-1 in AV-EVs (AV-PAI) complement plasma PlGF in predicting PE in a low-risk obstetric population. Eight hundred and forty-three prospectively banked plasma samples collected at 28 + 0 to 32 + 0 gestation weeks in the Neonatal and Obstetrics Risk Assessment (NORA) cohort study were assayed by sandwich ELISAs for plasma PlGF, CTB-TIMP1 and AV-PAI1. Nineteen patients subsequently developed PE 7.3 (±2.9) weeks later at a mean gestational age of 36.1 ± 3.5 weeks. The biomarkers were assessed for their predictive accuracy for PE using stepwise multivariate logistic regression analysis with Firth correction and Areas under the curve (AUC). To achieve 100% sensitivity in predicting PE, the cut-off for plasma PlGF, CTB-TIMP1 & AV-PAI1 were set at 1300 and
Databáze: Directory of Open Access Journals
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