Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran

Autor: Fariba BERENJI, Seyed Aliakbar SHAMSIAN, Marziyeh NOURI DALOEE, Seyed Hossein FATTAHI MASOOM, Elham MOGHADDAS
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Iranian Journal of Parasitology, Vol 14, Iss 1, Pp 52-58 (2019)
Druh dokumentu: article
ISSN: 1735-7020
2008-238X
Popis: Background: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. Methods: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1). Results: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified. Conclusion: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.
Databáze: Directory of Open Access Journals
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