A novel gene, CaATHB-12, negatively regulates fruit carotenoid content under cold stress in Capsicum annuum

Autor: Rui-Xing Zhang, Wen-Chao Zhu, Guo-Xin Cheng, Ya-Nan Yu, Quan-Hui Li, Saeed ul Haq, Fazal Said, Zhen-Hui Gong
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Food & Nutrition Research, Vol 64, Iss 0, Pp 1-16 (2020)
Druh dokumentu: article
ISSN: 1654-661X
DOI: 10.29219/fnr.v64.3729
Popis: Background: Carotenoids, the secondary metabolites terpenoids, are the largest factors that form the fruit color. Similar to flavonoids, they are not only safe and natural colorants of fruits but also play a role as stress response biomolecules. Methods: To study the contribution of the key genes in carotenoids biosynthesis, fruit-color formation, and in response to cold stress, we characterized the key regulatory factor CaATHB-12 from the HD-ZIP I sub-gene family members in pepper. Results: Cold stress enhanced carotenoid accumulation as compared with the normal condition. CaATHB-12 silencing through virus-induced gene silencing changed the fruit color by regulating the carotenoid contents. CaATHB-12 silencing increased the antioxidant enzyme activities in the fruits of pepper, exposed to cold stress, whereas CaATHB-12 overexpression decreased the activities of antioxidant enzymes in the transgenic Arabidopsis lines, exposed to cold stress, suggesting that CaATHB-12 is involved in the regulation of cold stress in the pepper fruits. Conclusion: Our research will provide insights into the formation of fruit color in pepper and contribution of CaATHB-12 in response to cold stress. Further study should be focused on the interaction between CaATHB-12 and its target gene.
Databáze: Directory of Open Access Journals