Bacterial Flora in Dental Cavities after Traditional and Alternative Methods for Cavity Preparation
| Autor: | Marina A. Darenskaya, Svetlana I. Tokmakova, Olga V. Bondarenko, Natalya V. Chechun, Alena A. Richter, Yuliya V. Lunitsyna, Evgeniy V. Mokrenko, Ivan S. Goncharov, Maria I. Suslikova, Larisa R. Kolesnikova, Isai M. Mikhalevich, Evgeniy V. Genich, Yulia O. Sinyova |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Biomedicine, Vol 12, Iss 3, Pp 412-416 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2158-0510 2158-0529 |
| DOI: | 10.21103/Article12(3)_OA12 |
| Popis: | The aim of the study was to establish the qualitative and quantitative composition of microbial flora of dental cavities (DCs) after traditional and alternative preparation, including different methods of isolating the working field. Material and Methods: Our study included 60 patients (mean age of 25.0±3.1 years) with DC Class 1 (Black's classification) without concomitant somatic pathology. To accomplish the study’s aim, 60 teeth were prepared. The main group (MG) consisted of 45 teeth prepared under absolute isolation with a rubber dam (RD). In the MG, 15 teeth were treated traditionally with a diamond bur with red and yellow markings (MG-1), 15 teeth were treated by ultrasound with a diamond tip (MG-2), and 15 teeth underwent hydrokinetic preparation (MG-3) with the Aquacut device (Velopex). In the comparison group (CG), which included 15 teeth, DCs were treated traditionally with a dental bur without the RD. The MG and CG were comparable in terms of the initial state of dental and microbiological status. The study of the qualitative and quantitative composition of the DC microflora showed that all DCs contained pathogenic β-hemolytic streptococcus in the CG. At the same time, the maximum number of cases (80%) was moderately contaminated.. MG-1 and MG-2, as in the CG, were characterized by the predominance of β-hemolytic streptococcus at the bottom of the treated cavity. At the same time, the incidence of moderate contamination decreased by 4 times and single contamination increased in the cultures to 80%, compared to the CG (P=0.001). In MG-3, β-hemolytic streptococcus also dominated in the bottom of DCs after RD setting. The number of colonies was single (66.7% of cases) and moderate (33.3% of cases), indicating a significant increase in single and a decrease in moderate infestation, compared to the CG (P |
| Databáze: | Directory of Open Access Journals |
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