Engineering of Synthetic Transcriptional Switches in Yeast

Autor:	Masahiro Tominaga, Akihiko Kondo, Jun Ishii
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	genetic switch transcriptional switch yeast synthetic transcription factor synthetic promoter directed evolution Science
Zdroj:	Life, Vol 12, Iss 4, p 557 (2022)
Druh dokumentu:	article
ISSN:	2075-1729
DOI:	10.3390/life12040557
Popis:	Transcriptional switches can be utilized for many purposes in synthetic biology, including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although to date such switches have mainly been developed in prokaryotes, those for eukaryotes are increasingly being reported as both rational and random engineering technologies mature. In this review, we describe yeast transcriptional switches with different modes of action and how to alter their properties. We also discuss directed evolution technologies for the rapid and robust construction of yeast transcriptional switches.
Databáze:	Directory of Open Access Journals
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