Effect of 8 weeks resistance training on sphingosine-1-phosphate level and gene expression of SK1 enzyme, isoforms of MHCs in skeletal muscles of male Wistar Rats
| Autor: | Banitalebi E (PhD), Ghatre Samani K (PhD), Mardani G (MSc), Soheili A (Pharm.D), Ansari Samani R (MSc), Teimori H (PhD) |
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| Jazyk: | perština |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | مجله دانشگاه علوم پزشکی گرگان, Vol 14, Iss 4, Pp 44-51 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1562-4765 2008-4080 |
| Popis: | Background and Objective: Sphingosine-1-phosphate (S1P) is involved in regulation of proliferation, differentiation, hypertrophy and anti-apoptosis and activation of satellite cells. This study was done to evaluated the effect of 8 weeks resistance training on sphingosine-1-phosphate level and gene expression of SK1 enzyme, isoforms of MHCs in skeletal muscles of male Wistar rats. Materials and Methods: This experimental study was done on Twenty four 8-week-old 190-250 gr male Wistar rats. The rats were allocated randomly into control (N=12) and training (N=12) groups. Resistance training was done using a 1 meter height ladder with 2 cm grid with an 85 degree incline, and weights attached to rat's tails. The content of S1P present in the chloroform layer was determined by means of high performance liquid chromatography (HPLC). Determination of relative mRNA expression was performed by Real-time PCR. Data were analyzed using SPSS-17, Kolmogorov-Smirnov and independent t-test. Results: Resistance exercise training increased the total content of S1P in FHL (fast-twitch) and soleus (slow-twitch) muscles in comparison with control group (P |
| Databáze: | Directory of Open Access Journals |
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