Th17/Treg balance and macrophage polarization ratio in lower extremity arteriosclerosis obliterans

Autor:	Zhen-Zhen Li, Min Liu, Xiong-Hui He, Zhen-Dong Liu, Zhan-Xiang Xiao, Hao Qian, You-Fei Qi, Cun-Chuan Wang
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	lower extremity arteriosclerosis regulatory t cells regulatory b cells inflammatory factors m1 macrophages m2 macrophages Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5
Zdroj:	Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 3, Pp 127-136 (2024)
Druh dokumentu:	article
ISSN:	2221-1691 2588-9222
DOI:	10.4103/apjtb.apjtb_782_23
Popis:	Objective: To explore the balance of peripheral blood T helper 17 cells/regulatory T cell (Th17/Treg) ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans (ASO). Methods: A rat model of lower extremity ASO was established, and blood samples from patients with lower extremity ASO before and after surgery were obtained. ELISA was used to detect interleukin 6 (IL-6), IL-10, and IL-17. Real-time RCR and Western blot analyses were used to detect Foxp3, IL-6, IL-10, and IL-17 expression. Moreover, flow cytometry was applied to detect the Th17/Treg ratio and M1/M2 ratio. Results: Compared with the control group, the iliac artery wall of ASO rats showed significant hyperplasia, and the concentrations of cholesterol and triglyceride were significantly increased (P
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/c9872cd5b42c4f8b8e9c2f80c1d7b112 Zobrazit plný text záznamu View record in DOAJ