| Autor: |
Derek M Murphy, Patrick G Buckley, Kenneth Bryan, Sudipto Das, Leah Alcock, Niamh H Foley, Suzanne Prenter, Isabella Bray, Karen M Watters, Desmond Higgins, Raymond L Stallings |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 4, Iss 12, p e8154 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0008154 |
| Popis: |
BACKGROUND:Neuroblastoma, a cancer derived from precursor cells of the sympathetic nervous system, is a major cause of childhood cancer related deaths. The single most important prognostic indicator of poor clinical outcome in this disease is genomic amplification of MYCN, a member of a family of oncogenic transcription factors. METHODOLOGY:We applied MYCN chromatin immunoprecipitation to microarrays (ChIP-chip) using MYCN amplified/non-amplified cell lines as well as a conditional knockdown cell line to determine the distribution of MYCN binding sites within all annotated promoter regions. CONCLUSION:Assessment of E-box usage within consistently positive MYCN binding sites revealed a predominance for the CATGTG motif (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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