Autor: |
Kern Rei Chng, Sock Hoai Chan, Amanda Hui Qi Ng, Chenhao Li, Apinya Jusakul, Denis Bertrand, Andreas Wilm, Su Pin Choo, Damien Meng Yew Tan, Kiat Hon Lim, Roy Soetinko, Choon Kiat Ong, Dan G. Duda, Simona Dima, Irinel Popescu, Chaisiri Wongkham, Zhu Feng, Khay Guan Yeoh, Bin Tean Teh, Puangrat Yongvanit, Sopit Wongkham, Vajaraphongsa Bhudhisawasdi, Narong Khuntikeo, Patrick Tan, Chawalit Pairojkul, Joanne Ngeow, Niranjan Nagarajan |
Jazyk: |
angličtina |
Rok vydání: |
2016 |
Předmět: |
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Zdroj: |
EBioMedicine, Vol 8, Iss C, Pp 195-202 (2016) |
Druh dokumentu: |
article |
ISSN: |
2352-3964 |
DOI: |
10.1016/j.ebiom.2016.04.034 |
Popis: |
Cholangiocarcinoma (CCA) is the primary cancer of the bile duct system. The role of bile duct tissue microbiomes in CCA tumorigenesis is unestablished. To address this, sixty primary CCA tumors and matched normals, from both liver fluke (Opisthorchis viverrini) associated (OVa, n = 28) and non-O. viverrini associated (non-OVa, n = 32) cancers, were profiled using high-throughput 16S rRNA sequencing. A distinct, tissue-specific microbiome dominated by the bacterial families Dietziaceae, Pseudomonadaceae and Oxalobacteraceae was observed in bile duct tissues. Systemic perturbation of the microbiome was noted in tumor and paired normal samples (vs non-cancer normals) for several bacterial families with a significant increase in Stenotrophomonas species distinguishing tumors vs paired normals. Comparison of parasite associated (OVa) vs non-associated (non-OVa) groups identified enrichment for specific enteric bacteria (Bifidobacteriaceae, Enterobacteriaceae and Enterococcaceae). One of the enriched families, Bifidobacteriaceae, was found to be dominant in the O. viverrini microbiome, providing a mechanistic link to the parasite. Functional analysis and comparison of CCA microbiomes revealed higher potential for producing bile acids and ammonia in OVa tissues, linking the altered microbiota to carcinogenesis. These results define how the unique microbial communities resident in the bile duct, parasitic infections and the tissue microenvironment can influence each other, and contribute to cancer. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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