| Autor: |
S. Bakshi, S. Sihi, D. N. Sengupta |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Biologia Plantarum, Vol 60, Iss 4, Pp 788-792 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1573-8264 |
| DOI: |
10.1007/s10535-016-0605-7 |
| Popis: |
Involvement of DNA polymerase (pol) enzymes in meiotic DNA repair has not been clearly understood in plants. DNA polymerase λ is involved in short patch DNA synthesis and base excision repair in both plants and animals. The presence and activity of the pol λ enzyme was studied in a protein isolated from spikelets during flower development stages of rice (Oryza sativa L.) and maize (Zea mays L.). Western blot analysis showed a 2- to 2.5-fold higher accumulation of pol λ in spikelets than in shoots. Assays of pol and in-gel activity showed the dideoxynucleotide triphosphate sensitive pol λ enzyme in spikelets of both the plants. An enhanced presence of the enzyme and its high activity suggests an active role of pol λ in meiotic recombination during microspore development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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