| Autor: |
Jeremy Adler, Ainsley Huang, Ingela Parmryd |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
SoftwareX, Vol 24, Iss , Pp 101570- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2352-7110 |
| DOI: |
10.1016/j.softx.2023.101570 |
| Popis: |
The plasma membrane that encloses cells is difficult to precisely delineate but this is often required for quantitation of fluorescence images. We have created an ImageJ macro that efficiently maps the plasma membrane based on a few imprecisely marked points as the user input, to generate a one-pixel-wide region of interest. A second macro makes measurements from the plasma membrane and optionally from additional regions of interest, offset both inwards and outwards from the plasma membrane. While we are interested in membrane order quantified by generalized polarization, any measurements from two or more channels could easily be implemented. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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