| Autor: |
Débora S. Lemos, Helen C. Beckert, Luana C. Oliveira, Fernanda C.B. Berti, Patricia M.M. Ozawa, Ingrid L.M. Souza, Silvio M. Zanata, Vânia C.S. Pankievicz, Thalita R. Tuleski, Emanuel M. Souza, Rosiane V. Silva, Pryscilla F. Wowk, Maria Luiza Petzl-Erler, Rodrigo C. Almeida, Gabriel Adelman Cipolla, Angelica B.W. Boldt, Danielle Malheiros |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
BBA Advances, Vol 2, Iss , Pp 100053- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-1603 |
| DOI: |
10.1016/j.bbadva.2022.100053 |
| Popis: |
Background: Celiac disease (CD) is an autoimmune disorder triggered by an abnormal immunological response to gluten ingestion and is associated with deregulated expression of cellular microRNAs (miRNAs) of the gut mucosa. It is frequently misdiagnosed as lactose intolerance (LI) due to symptom resemblance. Microvilli loss may be counteracted by a rigorous gluten-free diet (GFD). Aims: To identify altered extracellular vesicle miRNAs from plasma among CD patients on GFD (n=34), lactose intolerant individuals on restrictive diet (n=14) and controls (n=23), and to predict biological pathways in which these altered miRNAs may play a part. Methods: Five different small RNA samples of each group were pooled twice and then screened by new-generation sequencing. Four miRNAs were selected to be quantified by RT-qPCR in the entire sample. Results: The levels of four miRNAs – miR-99b-3p, miR-197-3p, miR-223-3p, and miR-374b-5p – differed between CD patients and controls (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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