Specialized functions and sexual dimorphism explain the functional diversity of the myeloid populations during glioma progression
| Autor: | Natalia Ochocka, Pawel Segit, Kamil Wojnicki, Salwador Cyranowski, Julian Swatler, Karol Jacek, Wiesława Grajkowska, Bozena Kaminska |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Cell Reports, Vol 42, Iss 1, Pp 111971- (2023) |
| Druh dokumentu: | article |
| ISSN: | 2211-1247 80337392 |
| DOI: | 10.1016/j.celrep.2022.111971 |
| Popis: | Summary: Malignant gliomas are aggressive, hard-to-treat brain tumors. Their tumor microenvironment is massively infiltrated by myeloid cells, mostly brain-resident microglia, bone marrow (BM)-derived monocytes/macrophages, and dendritic cells that support tumor progression. Single-cell omics studies significantly dissected immune cell heterogeneity, but dynamics and specific functions of individual subpopulations were poorly recognized. We use Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq) to precisely dissect myeloid cell identities and functionalities in murine GL261 gliomas. We demonstrate that the diversity of myeloid cells infiltrating gliomas is dictated by cell type and cell state. Glioma-activated microglia are the major source of cytokines attracting other immune cells, whereas BM-derived cells show the monocyte-to-macrophage transition in the glioma microenvironment. This transition is coupled with a phenotypic switch from the IFN-related to antigen-presentation and tumor-supportive gene expression. Moreover, we found sex-dependent differences in transcriptional programs and composition of myeloid cells in murine and human glioblastomas. |
| Databáze: | Directory of Open Access Journals |
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