Off-line ESI-MS/MS profiling of betalains and flavonoid glycosides isolated from (fruit) Opuntia stricta var. dillenii and (vegetable) Atriplex hortensis var. rubra by countercurrent chromatography
| Autor: | Thi Minh Thu Tran, Thanh Binh Nguyen, Peter Winterhalter, Gerold Jerz |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Vietnam Journal of Science, Technology and Engineering, Vol 64, Iss 1 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2525-2461 2615-9937 |
| DOI: | 10.31276/VJSTE.64(1).20-26 |
| Popis: | Opuntia stricta var. dillenii and Atriplex hortensis var. rubra, two plant species with abundant betalain and flavonoid content, were studied by high performance countercurrent chromatography (HPCCC) coupled with ESI-MS/ MS in an off-line detection process. Both CCC runs used tert.-butyl-methyl-ether/n-butanol/acetonitrile/water 1% trifluoroacetic acid (v/v/v/v, 2:2:1:5) as a biphasic solvent system within an elution-extrusion mode. The resulting fractions were scanned by an off-line sequential injection to ESI-MS/MS approach. The separation of Opuntia stricta var. dillenii extract showed a partial isolation of principal betalains (betanin) from their derivatives (betanidin, phyllocactin, feruloyl-betanin, betaxanthin), degraded products (hydroxyl-neobetanin, mono decarboxy-betanins), and flavonoid glycosides (isorhamnetin-rutinoside, kaempferol-rutinoside). In case of Atriplex hortensis var. rubra, this study confirmed the coexistence of several flavonoids (quercetin-Omalonyl glucoside, kaempferol-O-malonyl glucoside) and generated few pure pigment fractions (celosianin II) for structural elucidation. |
| Databáze: | Directory of Open Access Journals |
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