| Autor: |
Bing-ru ZHAO, Xue-feng FU, Ke-chuan TIAN, Xi-xia HUANG, Jiang DI, Yan BAI, Xin-ming XU, Yue-zhen TIAN, Wei-wei WU, Sulayman ABLAT, Wei-dan ZENG, Tulafu HANIKEZI |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Journal of Integrative Agriculture, Vol 18, Iss 10, Pp 2351-2360 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2095-3119 |
| DOI: |
10.1016/S2095-3119(19)62735-8 |
| Popis: |
As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD) (P=0.04) and live weight (LW) (P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW) (P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect