Optimization of Exopolysaccharide Production by Bacteria Strains Isolated from Brewery Wastewater Sludge collected from a Brewery Company in Uyo, Akwa Ibom State, Nigeria

Autor: I. O. Jacob, V. J. Umoh, U. E. Antia, B. E. Agbo, N. U. Stephen
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Applied Sciences and Environmental Management, Vol 28, Iss 8 (2024)
Druh dokumentu: article
ISSN: 2659-1502
2659-1499
Popis: Bacteria make expolysaccharides (EPS) in two basic forms; as firmly attached capsular-EPS and loosely attached slime-EPS. Hence, the objective of this paper was to investigate the optimization of exopolysaccharide (EPS) production by bacteria strains isolated from brewery wastewater sludge collected from a Brewery Company in Uyo, Akwa Ibom State, Nigeria using random mutagenesis. Bacterial isolates from brewery wastewater sludge samples were screened for EPS production using standard microbiological method. Six (6) best EPS-producing bacterial isolates were selected, identified molecularly as Bacillus altitudinis, Bacillus velezensis, Bacillus subtilis, Pseudomonas aeruginosa, Heyndrickxia oleronia, and Acinetobacter calcoaceticus, and used for optimization of EPS production after exposure to UV-irradiation and ethidium bromide (EtBr). In all the isolates, UV-irradiation caused the production of significantly (P
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