Trametes sp. as a Source of Biopolymer Cross-Linking Agents: Laccase Induced Gelation of Ferulated Arabinoxylans

Autor: Agustín Rascón-Chu, Georgina Vargas, Elizabeth Carvajal-Millán, Elisa Valenzuela-Soto, Rogerio R. Sotelo-Mundo, Ana Luisa Martínez, Alva Castillo
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Molecules, Vol 14, Iss 10, Pp 4159-4165 (2009)
Druh dokumentu: article
ISSN: 1420-3049
DOI: 10.3390/molecules14104159
Popis: This study examined the feasibility of using Trametes sp. grown under drought conditions to catalyze the covalent cross-linking of ferulated arabinoxylans. The rate of polymerization of ferulated arabinoxylans solutions at 0.5% (w/v) was dose dependent on the laccase concentration in the system. Above 1.2 nkat laccase/mg ferulated arabinoxylan, the rate of cross-linking reached a plateau, suggesting that the reaction reached saturation. For 2% (w/v) ferulated arabinoxylans treated with laccase (1.6 nkat/mg ferulated arabinoxylan), stable gels were formed after 4 h at 25 °C, with cross-linking contents (diferulic and triferulic acid) contents of 0.03 and 0.015 μg/mg ferulated arabinoxylan, respectively. This study demonstrated that Trametes sp. can be a source of biopolymer cross-linking enzymes like laccase.
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