| Autor: |
Muriel Jourdan, Julia Pingel, Arnaud Buhot, Thierry Livache, Jean-François Constant |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
AIMS Materials Science, Vol 2, Iss 4, Pp 473-483 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2372-0484 |
| DOI: |
10.3934/matersci.2015.4.473 |
| Popis: |
Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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