| Autor: |
Chen KANG, Juanjuan YAN, Chen JIANG, Jiayi ZHANG, Wanzhen XIE, Hongchen BI, Huiqiang LI |
| Jazyk: |
čínština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Zhongguo shipin weisheng zazhi, Vol 32, Iss 01, Pp 10-15 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1004-8456 |
| DOI: |
10.13590/j.cjfh.2020.01.002 |
| Popis: |
Objective To express the cashew nut allergen Ana o 2 and identify its immunological activity. Methods Ana o 2 gene was synthesized and inserted into the pET-28a (+) expression vector. The recombinant plasmid pET-28a (+) - Ana o 2 was constructed and sequenced. The correct recombinant plasmid was transformed into Rosetta (DE3), and the recombinant Ana o 2 protein was expressed and purified. The purified recombinant protein was identified by mass spectrometry. The immunological activity of the recombinant Ana o 2 protein was evaluated by enzyme-linked immunosorbent assay (ELISA) and protein immunoblot (Western blot). Results As determined by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of the recombinant protein was approximately 54 kD, which was consistent with the theoretical value. The recombinant protein was identified as Ana o 2 by mass spectrometry. ELISA result showed that the level of specific immunoglobulin E (sIgE) in cashew-allergic sera was significantly different from that in negative sera (t=2.44, P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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