N-Doped Carbon Quantum Dots as Fluorescent Bioimaging Agents
| Autor: | Shih-Fu Ou, Ya-Yun Zheng, Sin-Jen Lee, Shyi-Tien Chen, Chien-Hui Wu, Chien-Te Hsieh, Ruey-Shin Juang, Pei-Zhen Peng, Yi-Huang Hsueh |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Crystals, Vol 11, Iss 7, p 789 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2073-4352 63946262 |
| DOI: | 10.3390/cryst11070789 |
| Popis: | Graphene quantum dots, carbon nanomaterials with excellent fluorescence characteristics, are advantageous for use in biological systems owing to their small size, non-toxicity, and biocompatibility. We used the hydrothermal method to prepare functional N-doped carbon quantum dots (N-CQDs) from 1,3,6-trinitropyrene and analyzed their ability to fluorescently stain various bacteria. Our results showed that N-CQDs stain the cell septa and membrane of the Gram-negative bacteria Escherichia coli, Salmonellaenteritidis, and Vibrio parahaemolyticus and the Gram-positive bacteria Bacillus subtilis, Listeria monocytogenes, and Staphylococcus aureus. The optimal concentration of N-CQDs was approximately 500 ppm for Gram-negative bacteria and 1000 ppm for Gram-positive bacteria, and the exposure times varied with bacteria. N-Doped carbon quantum dots have better light stability and higher photobleaching resistance than the commercially available FM4-64. When excited at two different wavelengths, N-CQDs can emit light of both red and green wavelengths, making them ideal for bioimaging. They can also specifically stain Gram-positive and Gram-negative bacterial cell membranes. We developed an inexpensive, relatively easy, and bio-friendly method to synthesize an N-CQD composite. Additionally, they can serve as a universal bacterial membrane-staining dye, with better photobleaching resistance than commercial dyes. |
| Databáze: | Directory of Open Access Journals |
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