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Abeer ALrefai,1,2 Ashraf Dawood,1 Wafaa Shehata,3 Mohammed Elhelbawy,4 Nesreen Elhelbawy1 1Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Shebin Elkom City, Egypt; 2Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makah City, Saudi Arabia; 3Dermatology, Andrology & Sexually Transmitted Diseases (STDs) Department, Faculty of Medicine, Menoufia University, Shebin Elkom City, Egypt; 4Clinical Pathology Department, Faculty of Medicine, Menoufia University, Shebin Elkom City, EgyptCorrespondence: Nesreen Elhelbawy Tel +2 01069590533Fax +20482181009Email nesrin.elhelbawy@yahoo.comBackground: Psoriasis is a complex autoimmune multifactorial disease induced by interaction of environmental and genetic factors. This research aimed to clarify the association of NLRP3 (rs10754558) and PTPN22 (1858C/T) (rs2476601) polymorphisms with susceptibility to psoriasis.Methods: This case–control study involved 150 patients diagnosed with psoriasis and 100 age- and gender-matched apparently healthy individuals. NLRP3 (rs10754558) polymorphism was done by real time PCR and PTPN22 1858C/T (rs2476601) genotype was identified by tetra-primer amplification refractory mutation system-polymerase chain reaction (PCR) method.Results: The genotypes distribution of NLRP3 (rs10754558) were significantly associated with psoriasis (p< 0.0001). Whereas for PTPN22 (1858C/T) (rs2476601), no significance was found (p=0.09). NLRP3 (rs10754558) GC genotype revealed a significant association with psoriasis (p< 0.0001), mainly among male (p=0.004) patients with mild psoriasis (p=0.001) and affected extremities (p=0.0001).Conclusion: We can conclude that the NLRP3 (rs10754558) GC genotype may play a role in psoriasis susceptibility among male Egyptian populations with affected extremities. Future studies must evaluate its role in the prevention or the treatment of psoriasis.Keywords: psoriasis, NLRP3 (rs10754558), PTPN22 (1858C/T) (rs2476601), real time PCR |
