Effect of Pseudomonas aeruginosa Pure Exotoxin A on Mice WBC in Comparison with Human WBC Contaminated by Pseudomonas aeruginosa
| Autor: | M Naghmachi, A Sharifi, J Kohanteb |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Armaghane Danesh Bimonthly Journal, Vol 13, Iss 3, Pp 72-80 (2008) |
| Druh dokumentu: | article |
| ISSN: | 1728-6506 1728-6514 |
| Popis: | ABSTRACT Introduction & Objective: Pseudomonas aeruginosa is a gram negative bacterial. This bacterium is resistant to many antibiotics and chemical disinfectants. Pseudomonas aeruginosa is an opportunistic bacteria and caused infection in skin, external ear, upper respiratory tract, large intestine and is an important bacteria in nosocomial infections. It causes acute infection in burn disease. This bacterium can produce exotoxin A and effect on elongation factor II and can stop protein synthesis. The aim of this study was to evaluate the effect of exotoxin A on mice WBC and comparison of the results with human WBC that contaminated with Pseudomonas aeruginosa. Materials & Methods: This is an experimental study which was conducted in 1384 on burn disease patients referred to Shiraz Ghotbodin hospital. Sample that contaminated with PA was taken from these patients for WBC count and WBC differentiation. Sample was also taken from 100 burn patients without infection (50 male and 50 female). Toxigenic strain of PA103 was cultured on liquid media and used for purification of exotoxin A. This sample was injected to 50 mice (I.V) and after different incubation time, WBC was counted. Ten normal mice was used as control. Collected data analyzed by SPSS. Results: WBC count decreased in mice that received Pseudomonas aeruginosa exotoxin A in comparison with normal mice (P |
| Databáze: | Directory of Open Access Journals |
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