Sucrose, Lactose, and Xylitol Exposures Affect Biofilm Formation of Streptococcus mutans
| Autor: | Prawati Nuraini, Indah Listiana Kriswandini, Rini Devijanti Ridwan, Soetjipto |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Vol 21 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1519-0501 1983-4632 |
| Popis: | Objective: To determine the level of biofilm formation of S. mutans after being exposed to 5% sucrose, 8% lactose, or 1% xylitol. Material and Methods: This research was a laboratory-based experimental study with post-test only control group design. S. mutans was grown in test tubes containing tryptose soy broth (TSB) medium supplemented with 1% glucose. They were incubated at 37° C for 24 hours to grow the biofilms. The culture was then exposed to 5% sucrose, 8% lactose or 1% xylitol, incubated for 24 hours at 37° C, and examined using ELISA at a wavelength of 625 nm. The statistical analysis was performed using a one-way analysis of variance followed by the least significant difference test (α=0.05). Results: There were some differences in the biofilm formation of S. mutans after exposure to 5% sucrose, 8% lactose, or 1% xylitol (p0.05) between 8% lactose and 1% xylitol. Conclusion: Sucrose, lactose and xylitol can form biofilms and the formation of lactose biofilms is the same as xylitol. |
| Databáze: | Directory of Open Access Journals |
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