RNA Sequencing Unveils Very Small RNAs With Potential Regulatory Functions in Bacteria

Autor: Idrissa Diallo, Jeffrey Ho, David Lalaouna, Eric Massé, Patrick Provost
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Frontiers in Molecular Biosciences, Vol 9 (2022)
Druh dokumentu: article
ISSN: 2296-889X
DOI: 10.3389/fmolb.2022.914991
Popis: RNA sequencing (RNA-seq) is the gold standard for the discovery of small non-coding RNAs. Following a long-standing approach, reads shorter than 16 nucleotides (nt) are removed from the small RNA sequencing libraries or datasets. The serendipitous discovery of an eukaryotic 12 nt-long RNA species capable of modulating the microRNA from which they derive prompted us to challenge this dogma and, by expanding the window of RNA sizes down to 8 nt, to confirm the existence of functional very small RNAs (vsRNAs
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