Single-cell RNA sequencing-guided fate-mapping toolkit delineates the contribution of yolk sac erythro-myeloid progenitors
| Autor: | Y.X. Zhao, J.Y. Song, X.W. Bao, J.L. Zhang, J.C. Wu, L.Y. Wang, C. He, W. Shao, X.L. Bai, T.B. Liang, J.P. Sheng |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Cell Reports, Vol 42, Iss 11, Pp 113364- (2023) |
| Druh dokumentu: | article |
| ISSN: | 2211-1247 44368291 |
| DOI: | 10.1016/j.celrep.2023.113364 |
| Popis: | Summary: Erythro-myeloid progenitors of the yolk sac that originates during early embryo development has been suggested to generate tissue-resident macrophage, mast cell, and even endothelial cell populations from fetal to adult stages. However, the heterogeneity of erythro-myeloid progenitors (EMPs) is not well characterized. Here, we adapt single-cell RNA sequencing to dissect the heterogeneity of EMPs and establish several fate-mapping tools for each EMP subset to trace the contributions of different EMP subsets. We identify two primitive and one definitive EMP subsets from the yolk sac. In addition, we find that primitive EMPs are decoupled from definitive EMPs. Furthermore, we confirm that primitive and definitive EMPs give rise to microglia and other tissue-resident macrophages, respectively. In contrast, only Kit+ Csf1r− primitive EMPs generate endothelial cells transiently during early embryo development. Overall, our results delineate the contribution of yolk sac EMPs more clearly based on the single-cell RNA sequencing (scRNA-seq)-guided fate-mapping toolkit. |
| Databáze: | Directory of Open Access Journals |
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