A rapid and easy method for the DNA extraction from Cryptococcus neoformans

Autor: Sellami Hayet, Sellami Amira, Jarboui Mohammed, Mseddi Fatma, Ayadi Ali
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Biological Procedures Online, Vol 13, Iss 1, p 5 (2011)
Druh dokumentu: article
ISSN: 1480-9222
DOI: 10.1186/1480-9222-13-5
Popis: Abstract DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.
Databáze: Directory of Open Access Journals