Carnosine Catalyzes the Formation of the Oligo/Polymeric Products of Methylglyoxal
| Autor: | Tim Weigand, Benjamin Singler, Thomas Fleming, Peter Nawroth, Karel D. Klika, Christian Thiel, Hans Baelde, Sven F. Garbade, Andreas H. Wagner, Markus Hecker, Benito A. Yard, Albert Amberger, Johannes Zschocke, Claus P. Schmitt, Verena Peters |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 46, Iss 2, Pp 713-726 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1015-8987 1421-9778 44179855 |
| DOI: | 10.1159/000488727 |
| Popis: | Background/Aims: Reactive dicarbonyl compounds, such as methylglyoxal (MG), contribute to diabetic complications. MG-scavenging capacities of carnosine and anserine, which have been shown to mitigate diabetic nephropathy, were evaluated in vitro and in vivo. Methods: MG-induced cell toxicity was characterized by MTT and MG-H1-formation, scavenging abilities by Western Blot and NMR spectroscopies, cellular carnosine transport by qPCR and microplate luminescence and carnosine concentration by HPLC. Results: In vitro, carnosine and anserine dose-dependently reduced N-carboxyethyl lysine (CEL) and advanced glycation end products (AGEs) formation. NMR studies revealed the formation of oligo/polymeric products of MG catalyzed by carnosine or anserine. MG toxicity (0.3-1 mM) was dose-dependent for podocytes, tubular and mesangial cells whereas low MG levels (0.2 mM) resulted in increased cell viability in podocytes (143±13%, p |
| Databáze: | Directory of Open Access Journals |
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