| Autor: |
Sadeepa Y. Mallikarachchi, Nancy C. Rotich, Emma Gordon, Ann E. Hagerman |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Compounds, Vol 4, Iss 2, Pp 401-414 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2673-6918 |
| DOI: |
10.3390/compounds4020024 |
| Popis: |
Proteins that are amphiphilic and have low water solubility can self-assemble into nanoparticles useful in food science, pharmaceutical science, or biotechnology. However, protein nanoparticles exhibit drawbacks such as low stability unless the particles are coated. In the current study, tannic acid is the coating agent for nanoparticles synthesized from the protein zein. Tannic acid is a hydrolyzable tannin comprising a polyol esterified with galloyl residues. The nominal molecular formula of tannic acid (C76H52O46) suggests the material is decagalloyl glucose, obscuring its complex composition as a mixture of galloyl esters of glucose. We prepared hollow zein nanoparticles and coated them with tannic acid preparations that had short or long galloyl ester chains. The % α-helix of zein in nanoparticles is lower than in native zein but there is no effect of coating the particles with tannic acid. Interactions between the tannic acid and the zein slightly perturb the IR spectrum of the protein but there is no effect of galloyl chain length. We confirmed that tannic acid-coated particles have a more negative zeta potential, suggesting greater stability compared to uncoated particles. Coating with longer chain length tannic acid reduces particle diameter and tends to decrease polydispersity but does not change particle digestibility. Coating with shorter galloyl chain length tannic acid tends not to change particle diameter, reduces polydispersity of the particles, and stabilizes particles to enzymatic digestion. Tannic acid is a naturally occurring tunable coating for nanoparticles that can be used to adjust properties such as particle size, polydispersity, and digestibility for specific purposes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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