Genetic variability of blood groups in southern Brazil
| Autor: | Gabriela Waskow, Mirelen Moura de Oliveira Rodrigues, Gabriela Höher, Tor Onsten, Juliana Dal-Ri Lindenau, Marilu Fiegenbaum, Silvana Almeida |
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| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 43, Iss 2 |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| DOI: | 10.1590/1678-4685-gmb-2018-0327 |
| Popis: | Abstract We evaluated genetic variability among the blood groups Kell (c.578C > T and c.1790T > C), Kidd (c.838A > G), Duffy (c.125A > G, c.265C > T and c.1-67T > C), Diego (c.2561C > T), MNS (c.143T > C) and Rh (c.676G > C) in Rio Grande do Sul in southern Brazil. Genetic profiling from 382 volunteer blood donors was performed through allelic discrimination assays using a hydrolysis probe (TaqMan®) with a real-time PCR system. The sample was divided into two groups: Euro-Brazilian and Afro-Brazilian. A comparison with studies from other regions of Brazil and the 1000 Genomes Database showed significant differences for almost all polymorphisms evaluated in our population. Population differentiation between the Euro- and Afro-Brazilian groups was low (FST value 0.055). However, when each locus was evaluated individually, KEL*06 and FY*02N.01 allele frequencies were significantly higher in the Afro-Brazilian group than in the Euro-Brazilian group. Ethnic classification that uses phenotypic criteria to find blood units with rare antigens may be important when there is a need to detect blood units with an absence of Duffy antigens. There is also a greater probability of finding donors in the Afro-Brazilian group. Taken together, the data indicate strong European and African contributions to the gene pool, with intense admixture. |
| Databáze: | Directory of Open Access Journals |
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