Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy

Autor: Luca Villa, Pavlo Maksimov, Christine Luttermann, Mareen Tuschy, Alessia L. Gazzonis, Sergio A. Zanzani, Michele Mortarino, Franz J. Conraths, Maria Teresa Manfredi, Gereon Schares
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Parasites & Vectors, Vol 14, Iss 1, Pp 1-14 (2021)
Druh dokumentu: article
ISSN: 1756-3305
DOI: 10.1186/s13071-020-04557-6
Popis: Abstract Background Neospora caninum, a coccidian protozoan, represents an important cause of bovine abortion. Available N. caninum strains show considerable variation in vitro and in vivo, including different virulence in cattle. To which extent sexual recombination, which is possible in the intestines of domestic dogs and closely related carnivores as definitive hosts, contributes to this variation is not clear yet. Methods Aborted bovine foetuses were collected between 2015 and early 2019 from Italian Holstein Friesian dairy herds suffering from reproductive problems. A total of 198 samples were collected from 165 intensive farms located in Lombardy, northern Italy. N. caninum samples were subjected to multilocus-microsatellite genotyping using ten previously established microsatellite markers. In addition to our own data, those from a recent study providing data on five markers from other northern Italian regions were included and analysed. Results Of the 55 samples finally subjected to genotyping, 35 were typed at all or 9 out of 10 loci and their individual multilocus-microsatellite genotype (MLMG) determined. Linear regression revealed a statistically significant association between the spatial distance of the sampling sites with the genetic distance of N. caninum MLMGs (P
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