| Autor: |
Nattamon Niyomdecha, Sirinart Chomean, Chollanot Kaset |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Siriraj Medical Journal, Vol 75, Iss 1 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2228-8082 |
| DOI: |
10.33192/smj.v75i1.260524 |
| Popis: |
Objective: Evaluation of an antigen-based rapid test for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on artificially contaminated objects in comparison with a real-time reverse transcription-polymerase chain reaction (RT-qPCR) standard method. Materials and Methods: Artificial surface contamination with inactivated SARS-CoV-2 was tested on ten different objects comprising fruits and common materials. Three contamination levels with virus titers of 103, 104, and 105 pfu/100 μl were studied. Each object was spiked with 200 μl of virus suspension, samples were then collected by swabbing and evaluated by rapid antigen test and RT-qPCR. Additionally, 3- and 5-day contamination with SARSCoV‑2 at 105 pfu/100 μl was tested for some materials. Results: The detection rate obtained by the rapid antigen test with 103, 104, and 105 pfu/100 μl of SARS-CoV-2 was 10%, 90%, and 90%, respectively for the tested objects. RT‑qPCR showed a detection rate of 100% at all virus titers. Furthermore, both rapid antigen test and RT-qPCR were able to detect the 3- and 5-day extended contamination with SARS-CoV-2. Conclusion: The collected data suggests that the evaluated rapid antigen test is suitable for detection of SARS-CoV-2 adhered to non-human samples as a screening method. This simple method can reduce costs and turnaround time when compared to a standard molecular assay. It may be applied to enhance safety policies for COVID-19 prevention in public health and international export-businesses. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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