Academic examination stress: Effects on salivary cortisol, neuropeptide Y and interleukin-1β

Autor: Rabia Şemsi, Erdal Ergünol, Emre Kanad Er, Aylin Sepici Dinçel
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Aspects of Molecular Medicine, Vol 2, Iss , Pp 100030- (2023)
Druh dokumentu: article
ISSN: 2949-6888
DOI: 10.1016/j.amolm.2023.100030
Popis: Saliva is one of the preferred non-invasive body fluids for biomarker studies. This study aimed to investigate the possible alteration of stress biomarkers of the students before and after the examinations via salivary cortisol, neuropeptide Y (NPY), and Interleukin-1β (IL-1β) levels. Forty-four adults were included in the study and divided into groups of pre-examination (Group I) and post-examination (Group II). Salivary samples were collected between 8 and 9 a.m. before and after the exam, which ended at 5 p.m. by SARSTEDT saliva collection tubes. Participants were asked to soak the swab with saliva and take it out after 1 min. Swabs were kept at room temperature for 15–30 min and centrifuged for 10–15 min at 1500 g. Salivary cortisol (ng/mL), NPY (ng/mL), and IL-1β (pg/mL) levels were analyzed by Enzyme-linked immunosorbent assay (ELISA). The salivary cortisol, NPY and IL-1 β levels were significantly increased in Group II compared to Group I (9.65 ± 4.53, 6.37 ± 4.14, p
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