| Autor: |
Maya S Khater, Gauri R Kulkarni, Sagar S Khater, Haribhau Gholap, Rajendra Patil |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Materials Research Express, Vol 7, Iss 3, p 035005 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2053-1591 |
| DOI: |
10.1088/2053-1591/ab731a |
| Popis: |
The interaction of metal oxide nanoparticles (NPs) with cells and lipid bilayers is precarious in various fields such as antibacterial and drug or gene delivery. These require a strong control over NPs–cell interactions, an understanding of how the NPs surface impact their interaction with lipid bilayers and cells. Therefore, to elucidate Titanium dioxide (TiO _2 ) NPs of size 8–10 nm and 90–100 nm and their interaction with lipid bilayer of Escherichia coli and Staphylococcus aureus, we studied membrane potential, membrane permeability. Results of the traditional method of checking antibacterial activity - minimum inhibitory concentration (MIC) was co-related with change in membrane potential and membrane permeability. TiO _2 NPs 8–10 nm have profound action on depolarization of membrane potential of E. coli cells, while of S. aureus were not affected. TiO _2 NPs 90–100 nm have very less effect on membrane potential and permeability of both organisms. It is observed that there exists a strong co-relation between antibacterial activity of the TiO _2 NPs and change in the membrane potential and membrane permeability. These observations are also supported by membrane leakage test by estimation of protein, deoxyribonucleic acid (DNA) and potassium ion (K ^+ ) ion content. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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