| Autor: |
Mohammad Saleh Moulana, Sanya Haiaty, Ahad Bazmani, Roya Shabkhizan, Marziyeh Sadat Moslehian, Fatemeh Sadeghsoltani, Mostafa Mostafazadeh, Mohammad Reza Asadi, Mehdi Talebi, Zahra Jafari, Mohammad Reza Morovati, Mohammad Hosein Farzaei, Reza Rahbarghazi |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-10 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2662-7671 |
| DOI: |
10.1186/s12906-024-04432-2 |
| Popis: |
Abstract Colorectal cancer (CRC) is deadly anaplastic changes in the gastrointestinal tract with high-rate mortality. In recent years, the application of phytocompounds has been extended along with different therapeutic protocols. Here, we monitored the effects of Thymoquinone (TQ) on autophagy via mitochondrial function after modulation of the Wnt/β-catenin signaling pathway. Human colorectal adenocarcinoma HT-29 cells were treated with TQ (60 µM) and 15 µM Wnt3a inhibitor (LGK974) for 48 h. The survival rate was evaluated using an MTT assay. The expression of Wnt-related factors (c-Myc, and Axin), angiogenesis (VE-Cadherin), and mitophagy-related factors (PINK1, OPTN) was assessed using real-time PCR assay. Protein levels of autophagy factors (Beclin-1, LC3, and P62) were monitored using western blotting. Using flow cytometry analysis, the intracellular accumulation of Rhodamine 123 was evaluated. The migration properties were analyzed using a scratch wound healing assay. Data indicated that TQ can reduce the viability of HT-29 cells compared to the control cells (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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