Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization

Autor: Fatou Benoit, Salzet Michel, Fournier Isabelle
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: The EuroBiotech Journal, Vol 3, Iss 2, Pp 97-104 (2019)
Druh dokumentu: article
ISSN: 2564-615X
DOI: 10.2478/ebtj-2019-0011
Popis: We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification.
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