Highly dynamic temporal changes of TSPY gene copy number in aging bulls.
| Autor: | Olutobi A Oluwole, Kiana Mahboubi, Laura A Favetta, Tamas Revay, Tom Kroetsch, William Allan King |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 12, Iss 5, p e0178558 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1932-6203 28859316 |
| DOI: | 10.1371/journal.pone.0178558 |
| Popis: | The Y-chromosomal TSPY gene is one of the highest copy number mammalian protein coding gene and represents a unique biological model to study various aspects of genomic copy number variations. This study investigated the age-related copy number variability of the bovine TSPY gene, a new and unstudied aspect of the biology of TSPY that has been shown to vary among cattle breeds, individual bulls and somatic tissues. The subjects of this prospective 30-month long study were 25 Holstein bulls, sampled every six months. Real-time quantitative PCR was used to determine the relative TSPY copy number (rTSPY CN) and telomere length in the DNA samples extracted from blood. Twenty bulls showed an altered rTSPY CN after 30 months, although only 9 bulls showed a significant change (4 significant increase while 5 significant decrease, P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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