| Autor: |
C. Charlier, N. Cox, S. M. Prud'homme, A. Geffard, J.-M. Nuzillard, B. Luy, G. Lippens |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Magnetic Resonance, Vol 2, Pp 619-627 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2699-0016 |
| DOI: |
10.5194/mr-2-619-2021 |
| Popis: |
The heteronuclear single quantum correlation (HSQC) experiment developed by Bodenhausen and Ruben (1980) in the early days of modern nuclear magnetic resonance (NMR) is without a doubt one of the most widely used experiments, with applications in almost every aspect of NMR including metabolomics. Acquiring this experiment, however, always implies a trade-off: simplification versus resolution. Here, we present a method that artificially lifts this barrier and demonstrate its application towards metabolite identification in a complex mixture. Based on the measurement of clean in-phase and clean anti-phase (CLIP/CLAP) HSQC spectra (Enthart et al., 2008), we construct a virtually decoupled HSQC (vd-HSQC) spectrum that maintains the highest possible resolution in the proton dimension. Combining this vd-HSQC spectrum with a J-resolved spectrum (Pell and Keeler, 2007) provides useful information for the one-dimensional proton spectrum assignment and for the identification of metabolites in Dreissena polymorpha (Prud'homme et al., 2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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