Betamethasone improved near‐term neonatal lamb lung maturation in experimental maternal asthma

Autor: Joshua L. Robinson, Andrea J. Roff, Sarah J. Hammond, Jack R. T. Darby, Ashley S. Meakin, Stacey L. Holman, Andrew Tai, Tim J. M. Moss, Catherine G. Dimasi, Sarah M. Jesse, Michael D. Wiese, Andrew N. Davies, Beverly S. Muhlhausler, Robert J. Bischof, Megan J. Wallace, Vicki L. Clifton, Janna L. Morrison, Michael J. Stark, Kathryn L. Gatford
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Experimental Physiology, Vol 109, Iss 11, Pp 1967-1979 (2024)
Druh dokumentu: article
ISSN: 1469-445X
0958-0670
DOI: 10.1113/EP091997
Popis: Abstract Maternal asthma is associated with increased rates of neonatal lung disease, and fetuses from asthmatic ewes have fewer surfactant‐producing cells and lower surfactant‐protein B gene (SFTPB) expression than controls. Antenatal betamethasone increases lung surfactant production in preterm babies, and we therefore tested this therapy in experimental maternal asthma. Ewes were sensitised to house dust mite allergen, and an asthmatic phenotype induced by fortnightly allergen lung challenges; controls received saline. Pregnant asthmatic ewes were randomised to receive antenatal saline (asthma) or 12 mg intramuscular betamethasone (asthma+beta) at 138 and 139 days of gestation (term = 150 days). Lambs were delivered by Caesarean section at 140 days of gestation and ventilated for 45 min before tissue collection. Lung function and structure were similar in control lambs (n = 16, 11 ewes) and lambs from asthma ewes (n = 14, 9 ewes). Dynamic lung compliance was higher in lambs from asthma+beta ewes (n = 12, 8 ewes) compared to those from controls (P = 0.003) or asthma ewes (P = 0.008). Lung expression of surfactant protein genes SFTPA (P = 0.048) and SFTPB (P
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