Magnetic Stirring Device for Limiting the Sedimentation of Cells inside Microfluidic Devices

Autor: Sebastian Cremaschini, Noemi Torriero, Chiara Maceri, Maria Poles, Sarah Cleve, Beatrice Crestani, Alessio Meggiolaro, Matteo Pierno, Giampaolo Mistura, Paola Brun, Davide Ferraro
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Sensors, Vol 24, Iss 15, p 5014 (2024)
Druh dokumentu: article
ISSN: 1424-8220
20843879
DOI: 10.3390/s24155014
Popis: In experiments considering cell handling in microchannels, cell sedimentation in the storage container is a key problem because it affects the reproducibility of the experiments. Here, a simple and low-cost cell mixing device (CMD) is presented; the device is designed to prevent the sedimentation of cells in a syringe during their injection into a microfluidic channel. The CMD is based on a slider crank device made of 3D-printed parts that, combined with a permanent magnet, actuate a stir bar placed into the syringe containing the cells. By using A549 cell lines, the device is characterized in terms of cell viability (higher than 95%) in different mixing conditions, by varying the oscillation frequency and the overall mixing time. Then, a dedicated microfluidic experiment is designed to evaluate the injection frequency of the cells within a microfluidic chip. In the presence of the CMD, a higher number of cells are injected into the microfluidic chip with respect to the static conditions (2.5 times), proving that it contrasts cell sedimentation and allows accurate cell handling. For these reasons, the CMD can be useful in microfluidic experiments involving single-cell analysis.
Databáze: Directory of Open Access Journals
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