| Autor: |
Silvia Pascual-Sabater, Giulia Raimondi, Ana Mato-Berciano, Eva C. Vaquero, Fabio Ausania, Cristina Fillat |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 2, Iss 4, Pp 101017- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2021.101017 |
| Popis: |
Summary: Patient-derived organoids (PDOs) have shown the potential to reflect patient sensitivity to chemotherapeutic or targeted drugs. Recently, we showed that organoid models can also serve as a platform to screen for selectivity and potency of oncolytic adenoviruses (OAds). In this protocol, we describe the steps for tumor organoid adenoviral infection and functional assessment of patient-specific responses to OAds. We provide methods to determine OAd relative efficacy by evaluation of PDO viability after infection and adenoviral replication within cancer cells.For complete details on the use and execution of this protocol, please refer to Raimondi et al. (2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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