| Autor: |
Anita Rani, Vineet Kumar, Shruti Shukla, Priyamvada Jha, Trupti Tayalkar, Priyanka Mittal |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Journal of Agriculture and Food Research, Vol 2, Iss , Pp 100065- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1543 |
| DOI: |
10.1016/j.jafr.2020.100065 |
| Popis: |
Genetic removal of Kunitz trypsin inhibitor (KTI), a proteinaceous anti-nutritional factor, in soybean is anticipated to cause loss in protein content. In the present study, marker-assisted survey of 180 recombinant inbred lines (RILs) developed from the cross KTI + ve (‘SL525’) × KTI-free parent (‘NRC101’) in tandem with quantification of protein content led to the identification of KTI-free RILs with normal-protein content. SDS-PAGE analysis of KTI-free parent, KTI + ve parent, low-protein KTI-free, and 2 normal-protein KTI-free RILs showed random increase and decrease in the concentration of different subunits of glycinin (11S), β-conglycinin (7S) and other storage protein in the normal-protein KTI-free RILs compared to KTI + ve parent. KTI-free RILs with normal-protein content showed higher concentration of acidic and basic subunits of 11S and β-subunit of 7S fraction compared to the low-protein KTI-free RIL and KTI-free parent. The study suggested that it is possible to breed KTI-free soybean without reducing the protein content. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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