Imobilização de lacase de Aspergillus sp. em quitosana e sua aplicação na bioconversão de fenóis em reatores de leito fixo
| Autor: | Everton Skoronski, Mylena Fernandes, Agenor Furigo Júnior, Carlos Henrique Lemos Soares, Jair Juarez João |
|---|---|
| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 37, Iss 2, Pp 215-220 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.5935/0100-4042.20140037 |
| Popis: | The immobilization of laccase on chitosan by cross-linking and application of the immobilized laccase in the bioconversion of phenolic compounds in batch and fixed bed reactors were studied. The process for immobilization of enzyme was optimized using a rotational central composite design. The optimized conditions to generate immobilized laccase with maximal activity were determined to be a glutaraldehyde concentration of 1.0% (v/v), a pH of 6.0, an immobilization time of 5.0 hours and an enzyme concentration of 5.2 g L-1. In packed bed reactors, the activity of the immobilized enzyme is maintained for a longer time in the bioconversion of 2,6-dimethoxyphenol than in the bioconversion of syringaldazine. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|