A novel use of a needle-free injection system for improved nucleic acid delivery and expression in vivo
| Autor: | Nathan Liu, Hani Abd-Ul-Salam, Noémie Joannette-Lafrance, Jingjing Li, Karim Menassa, Monzur Murshed |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 75, Iss 2, Pp 65-70 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2023-0015 |
| Popis: | Transfection, a nonviral method of nucleic acid delivery, often exhibits poor efficiency in vivo. The needle-based in vivo delivery of transfection reagents can be invasive. Here, we report a noninvasive protocol for in vivo gene delivery via the needle-free MED-JET H4 MULTIJET (MJH4M) device using both “home-made” glucose-based and commercial transfection reagents. The objective of this study was to compare the relative transfection efficiencies of the needle-free system to that of the needle-based delivery method. We observed a 15-fold increase in transfection efficiency using the needle-free MJH4M device when compared to the needle-based delivery method. The highest transfection efficiency was achieved using a 5% glucose solution as the delivery vehicle. |
| Databáze: | Directory of Open Access Journals |
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