Characterization of bolting behaviour in garlic (Allium sativum) using SNP
| Autor: | YADWINDER KAUR, R K DHALL, PRITI SHARMA |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Indian Journal of Agricultural Sciences, Vol 90, Iss 1 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0019-5022 2394-3319 |
| DOI: | 10.56093/ijas.v90i1.98556 |
| Popis: | The present research was conducted to evaluate the bolting (incomplete bolters and non-bolters) behaviour in garlic (Allium sativum L.) accessions collected from different geographical areas. A total of 96 genotypes collected from different sources were used for genotype sequencing to identify SNP but only 73 genotypes among them showed good amplification. A primer (AsLFY) was designed by using reference of already designed gene specific primer (gALFY-2) by Rotem et al. (2007). After amplification, sequencing of these 73 genotypes was done; SNP (CCGTCG) was at 301 bp from initiation codon, i.e. G for non-bolters genotypes and A for incomplete bolter genotypes. Thus, the identified SNP would help in detecting incomplete bolter and non-bolter genotypes at seedling stage which can be useful for garlic breeding programmes. |
| Databáze: | Directory of Open Access Journals |
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