Autor: |
Zurong Wan, Jinghua Gu, Uthra Balaji, Linda Bojmar, Henrik Molina, Søren Heissel, Alexandra E. Pagano, Christopher Peralta, Lee Shaashua, Dorina Ismailgeci, Hope K. Narozniak, Yi Song, William R. Jarnagin, David P. Kelsen, Jaqueline Bromberg, Virginia Pascual, Haiying Zhang |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
Journal of Extracellular Biology, Vol 3, Iss 7, Pp n/a-n/a (2024) |
Druh dokumentu: |
article |
ISSN: |
2768-2811 |
DOI: |
10.1002/jex2.167 |
Popis: |
Abstract Circulating extracellular vesicles and particles (EVPs) are being investigated as potential biomarkers for early cancer detection, prognosis, and disease monitoring. However, the suboptimal purity of EVPs isolated from peripheral blood plasma has posed a challenge of in‐depth analysis of the EVP proteome. Here, we compared the effectiveness of different methods for isolating EVPs from healthy donor plasma, including ultracentrifugation (UC)‐based protocols, phosphatidylserine‐Tim4 interaction‐based affinity capture (referred to as “PS”), and several commercial kits. Modified UC methods with an additional UC washing or size exclusion chromatography step substantially improved EVP purity and enabled the detection of additional proteins via proteomic mass spectrometry, including many plasma membrane and cytoplasmic proteins involved in vesicular regulation pathways. This improved performance was reproduced in cancer patient plasma specimens, resulting in the identification of a greater number of differentially expressed EVP proteins, thus expanding the range of potential biomarker candidates. However, PS and other commercial kits did not outperform UC‐based methods in improving plasma EVP purity. PS yielded abundant contaminating proteins and a biased enrichment for specific EVP subsets, thus unsuitable for proteomic profiling of plasma EVPs. Therefore, we have optimized UC‐based protocols for circulating EVP isolation, which enable further in‐depth proteomic analysis for biomarker discovery. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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